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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Organoplate Graft 48 Uf Chip Design, supplied by Mimetas Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/organoplate graft 48 uf chip design/product/Mimetas Inc
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organoplate graft 48 uniflow uf chip design  (Mimetas Inc)
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Organoplate Graft 48 Uniflow Uf Chip Design, supplied by Mimetas Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Custom Designed Chip, supplied by Formlabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom designed chip/product/Formlabs Inc
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Microfluidic Chip Design, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfluidic chip design/product/Autodesk Inc
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microfluidic chip design - by Bioz Stars, 2026-06
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Chip Design, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip design - by Bioz Stars, 2026-06
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Custom Designed Oncoarray Chip Illumina Oncoarray 534k, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom-designed oncoarray-chip illumina oncoarray 534k/product/Illumina Inc
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custom-designed oncoarray-chip illumina oncoarray 534k - by Bioz Stars, 2026-06
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organoplate 2 lane 48 uf chip design  (Mimetas Inc)
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
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https://www.bioz.com/result/organoplate 2 lane 48 uf chip design/product/Mimetas Inc
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organoplate 2 lane 48 uf chip design - by Bioz Stars, 2026-06
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inverse design fiber-to-chip couplers for the o- and c-bands  (Corning Life Sciences)
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Corning Life Sciences inverse design fiber-to-chip couplers for the o- and c-bands
Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate <t>Graft</t> <t>48</t> UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm
Inverse Design Fiber To Chip Couplers For The O And C Bands, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverse design fiber-to-chip couplers for the o- and c-bands/product/Corning Life Sciences
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inverse design fiber-to-chip couplers for the o- and c-bands - by Bioz Stars, 2026-06
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Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate Graft 48 UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm

Journal: Fluids and Barriers of the CNS

Article Title: Building the blood-brain barrier: a scalable self-assembling 3D model of the brain microvasculature under unidirectional flow

doi: 10.1186/s12987-026-00765-x

Figure Lengend Snippet: Brain endothelial cells, pericytes, and astrocytes self-assemble into vascular networks in the OrganoPlate Graft 48 UF. ( A ) The OrganoPlate Graft 48 UF is based on a modified 384-well microtiter plate format where long wells are created by merging 3 wells of the titer plate together to link perfusion channels fluidically. It contains 48 chips that can be used to model a BBB vascular network. Each chip comprises a gel chamber (blue) and two perfusion lanes (red, orange). ( B ) Human brain microvascular endothelial cells (HBMECs), astrocytes, and pericytes are embedded in an extracellular matrix gel and seeded to the gel chamber of each chip. Next, HBMECs are seeded in the adjacent perfusion lanes and form vessel-like structures against the extracellular matrix gel. The cells self-assemble into a 3D BBB network. ( C ) Perfusion is generated by placing the OrganoPlate Graft 48 UF on a rocker platform that induces gravity-driven fluid flow. When the angle of inclination is negative, medium is routed through the short channel only and split down two paths: (1) through the gel chamber and towards the long channel (bottom to top of the gel chamber) and (2) continuing through the short channel. ( D ) When the angle of inclination is positive, medium is flowing through the short channel in opposite direction, dividing into similar flow patterns from bottom to top of the gel chamber into the long channel and some flow continuing through the short channel. Red arrow indicates flow direction. ( E ) Phase contrast pictures of BBB monocultures and co-cultures obtained on day 7 after seeding. Scale bar = 200 µm

Article Snippet: The OrganoPlate Graft 48 UF chip design includes a graft chamber lined by a long and a short channel.

Techniques: Modification, Generated